Search results for "Vector Biology"

showing 5 items of 5 documents

AAV vector-mediated overexpression of CB1 cannabinoid receptor in pyramidal neurons of the hippocampus protects against seizure-induced excitoxicity.

2010

The CB1 cannabinoid receptor is the most abundant G-protein coupled receptor in the brain and a key regulator of neuronal excitability. There is strong evidence that CB1 receptor on glutamatergic hippocampal neurons is beneficial to alleviate epileptiform seizures in mouse and man. Therefore, we hypothesized that experimentally increased CB1 gene dosage in principal neurons would have therapeutic effects in kainic acid (KA)-induced hippocampal pathogenesis. Here, we show that virus-mediated conditional overexpression of CB1 receptor in pyramidal and mossy cells of the hippocampus is neuroprotective and moderates convulsions in the acute KA seizure model in mice. We introduce a recombinant a…

Central Nervous SystemCannabinoid receptormedicine.medical_treatmentHippocampuslcsh:MedicineHippocampal formationHippocampuschemistry.chemical_compoundMiceReceptor Cannabinoid CB1Neurobiology of Disease and RegenerationTransgeneslcsh:ScienceNeuronsRecombination GeneticMultidisciplinaryBehavior AnimalNeuromodulationmusculoskeletal neural and ocular physiologyfood and beveragesNeurochemistryGenomicsGene TherapyDependovirusEndocannabinoid systemCell biologyFunctional GenomicsNeurologyHomeostatic MechanismsMedicinelipids (amino acids peptides and proteins)Viral VectorsNeurochemicalsGenetic EngineeringResearch ArticleBiotechnologyKainic acidGenetic VectorsGreen Fluorescent ProteinsNeurophysiologyBiologyMicrobiologyVector BiologyGlutamatergicGenomic MedicineSeizuresmedicineGeneticsAnimalsBiologyEpilepsyIntegrasesDentate gyruslcsh:RMolecular biologyMice Inbred C57BLchemistryGene Expression Regulationnervous systemGenetics of DiseaseSynapseslcsh:QCannabinoidGene FunctionMolecular NeuroscienceAnimal GeneticsTransgenicsNeuroscienceEndocannabinoidsPLoS ONE
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Impact of vector dispersal and host-plant fidelity on the dissemination of an emerging plant pathogen

2012

International audience; Dissemination of vector-transmitted pathogens depend on the survival and dispersal of the vector and the vector's ability to transmit the pathogen, while the host range of vector and pathogen determine the breath of transmission possibilities. In this study, we address how the interaction between dispersal and plant fidelities of a pathogen (stolbur phytoplasma tuf-a) and its vector (Hyalesthes obsoletus: Cixiidae) affect the emergence of the pathogen. Using genetic markers, we analysed the geographic origin and range expansion of both organisms in Western Europe and, specifically, whether the pathogen's dissemination in the northern range is caused by resident vecto…

0106 biological sciencesRange (biology)Population DynamicsPopulation geneticslcsh:Medicine01 natural sciencessanté des plantesphytoplasme du stolburPhyletic PatternsVitisUrtica dioicahyalesthes obsoletuslcsh:SciencePathogenpathologie végétalePhylogenybactérie0303 health sciencesMultidisciplinaryEcologyEcologystolburUrtica dioicaAgricultureBiodiversityHost-Pathogen InteractionPhytoplasmaépidémiologieinsecte vecteuragent pathogèneResearch ArticleDNA BacterialGenetic MarkersPhytoplasmaEvolutionary ProcessesPhytopathology and phytopharmacyEmergenceBiologyDNA MitochondrialMicrobiologyVector Biology03 medical and health sciencesmollicute phytopathogèneIntegrated ControlintéractionEvolutionary SystematicsParasite EvolutionBiologyHybridizationMicrobial Pathogens030304 developmental biologyPlant DiseasesEvolutionary BiologyPopulation BiologyHost (biology)lcsh:Rtransmission de la maladiebiology.organism_classificationPhytopathologie et phytopharmacievariation génétiqueOrganismal Evolution[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacyEmerging Infectious DiseasesVector (epidemiology)Microbial EvolutionBiological dispersallcsh:QParasitologyPest ControlPopulation EcologyZoologyEntomologyPopulation Genetics010606 plant biology & botanyCoevolution
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Intracerebral Borna Disease Virus Infection of Bank Voles Leading to Peripheral Spread and Reverse Transcription of Viral RNA

2011

Bornaviruses, which chronically infect many species, can cause severe neurological diseases in some animal species; their association with human neuropsychiatric disorders is, however, debatable. The epidemiology of Borna disease virus (BDV), as for other members of the family Bornaviridae, is largely unknown, although evidence exists for a reservoir in small mammals, for example bank voles (Myodes glareolus). In addition to the current exogenous infections and despite the fact that bornaviruses have an RNA genome, bornavirus sequences integrated into the genomes of several vertebrates millions of years ago. Our hypothesis is that the bank vole, a common wild rodent species in traditional B…

Disease reservoirviruksetEpidemiologyanimal diseasesvirusesVeterinary MicrobiologyUrineVirus ReplicationMOUSE413 Veterinary sciencePolymerase Chain ReactionFecesInfectious Diseases of the Nervous SystemZoonosesBRAINBorna disease virusAntigens Viralbornavirus0303 health sciencesBorna diseaseMultidisciplinarybiologyArvicolinaeZoonotic DiseasesQR3. Good healthBank voleInfectious DiseasesBorna Virus InfectionVeterinary DiseasesArvicolinaeMedical MicrobiologyWILD RODENTSRNA ViralMedicineViral VectorsVeterinary PathologyResearch ArticleEXPRESSIONNeurovirulenceScienceUrinary BladdereducationANTIGENMicrobiologyVector BiologyInfectious Disease EpidemiologyVirusRATSPERSISTENT03 medical and health sciencesVirologyPeripheral Nervous SystemAnimalsHumansViral Nucleic AcidViral sheddingBiologyDisease Reservoirs030304 developmental biology030306 microbiologySTRAINSCENTRAL-NERVOUS-SYSTEMReproducibility of ResultsReverse TranscriptionVeterinary Virologybiology.organism_classificationVirologyViral ReplicationReverse transcriptaseMODELAnimals NewbornViral replicationBorna DiseaseAntibody FormationDNA ViralVeterinary ScienceViral Transmission and InfectionPLoS ONE
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A Wickerhamomyces anomalus Killer Strain in the Malaria Vector Anopheles stephensi

2013

The yeast Wickerhamomyces anomalus has been investigated for several years for its wide biotechnological potential, especially for applications in the food industry. Specifically, the antimicrobial activity of this yeast, associated with the production of Killer Toxins (KTs), has attracted a great deal of attention. The strains of W. anomalus able to produce KTs, called "killer" yeasts, have been shown to be highly competitive in the environment. Different W. anomalus strains have been isolated from diverse habitats and recently even from insects. In the malaria mosquito vector Anopheles stephensi these yeasts have been detected in the midgut and gonads. Here we show that the strain of W. a…

EpidemiologyApplied Microbiologylcsh:MedicineDisease VectorsMosquitoesMedicine and Health Scienceslcsh:Science0303 health sciencesFungal proteinMultidisciplinaryEcologybiologyAnophelesAntimicrobial3. Good healthInsectsFemaleResearch ArticleBiotechnologyArthropodaWickerhamomyces anomalusSaccharomyces cerevisiaeMycologyMicrobiologyVector BiologyMicrobial EcologyMicrobiologyFungal Proteins03 medical and health sciencesMicrobial ControlAnophelesparasitic diseasesAnimalsAnopheles stephensiKiller yeast030304 developmental biology030306 microbiologylcsh:RfungiOrganismsFungiBiology and Life SciencesMycotoxinsbiology.organism_classificationInvertebratesYeastYeastInsect VectorsMalariaSaccharomycetaleslcsh:QParasitologyZoologyEntomologyPLoS ONE
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Environmental change and disease dynamics: effects of intensive forest management on Puumala hantavirus infection in boreal bank vole populations.

2012

Intensive management of Fennoscandian forests has led to a mosaic of woodlands in different stages of maturity. The main rodent host of the zoonotic Puumala hantavirus (PUUV) is the bank vole (Myodes glareolus), a species that can be found in all woodlands and especially mature forests. We investigated the influence of forest age structure on PUUV infection dynamics in bank voles. Over four years, we trapped small mammals twice a year in a forest network of different succession stages in Northern Finland. Our study sites represented four forest age classes from young (4 to 30 years) to mature (over 100 years) forests. We show that PUUV-infected bank voles occurred commonly in all forest age…

0106 biological sciencesViral DiseasesEpidemiologyPopulation Dynamicslcsh:MedicineWoodlandWildlife01 natural sciencesPopulation densityPuumala virusTreesZoonoseslcsh:ScienceSmall Animals0303 health sciencesMultidisciplinarybiologyEcologyEcologyArvicolinaeZoonotic DiseasesBank voleMammalogyInfectious DiseasesArvicolinaeVeterinary DiseasesHemorrhagic Fever with Renal SyndromeMedicinePuumala virusTemperate rainforestResearch ArticleHantavirusHantavirus InfectionsAnimal TypeseducationForest management010603 evolutionary biologyMicrobiologyVector BiologyInfectious Disease Epidemiology03 medical and health sciencesVirologyAnimalsDisease DynamicsBiology030304 developmental biologyPopulation Biologylcsh:RfungiHemorrhagic Fevers15. Life on landbiology.organism_classificationEmerging Infectious Diseasesta1181lcsh:QVeterinary Science3111 BiomedicinePopulation EcologyHantavirus InfectionZoologyPloS one
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